ABSTRACT
Objective:To explore the expression and biological functions of miR-346 in nasopharyngeal carcinoma.Methods:63 cases nasopharyngeal carcinoma tissue and 34 cases nasopharyngeal non-cancer tissues were collected,the miR-346 expression were detected by Real-time PCR between nasopharyngeal carcinoma tissue and nasopharyngeal non-cancer tissues,6 strains of human nasopharyngeal carcinoma cell lines and 1 strain of normal nasopharyngeal epithelial cell immortalized NP69.Two cell lines with middle expression levels in human nasopharyngeal carcinoma cells lines were selected,and transfected into miR-346 inhibitor,the control group (NC group) were with negative control plasmid transfection,miR-346 expression in two groups were detected by Real-time PCR,the proliferation,apoptosis were detected by Brdu-ELISA and flow cytometry,the migration and invasion were detected by Transwell Chambers experiments.Results:Compared with the nasopharyngeal non-cancer tissues,the miR-346 expression in nasopharyngeal carcinoma tissues was significantly increased (P =0.000);compared with the normal nasopharyngeal epithelial cells NP69.the miR-346 expression in human nasopharyngeal carcinoma cell lines was significantly increased (P＜0.05).The CNE-1 and CNE-2 were chose,after the miR-346 inhibitor transfection,the miR-346 expression were significantly lower compared with NC group,the difference was statistically significant (P＜0.05).The proliferation of two kinds of nasopharyngeal carcinoma cell CNE-1 and CNE-2 were restrained after transfection,the difference showed statistically significant 3 days after transfection (P ＜ 0.05).The apoptosis increased significantly,and the migration cell numbers and invasion cell numbers decreased significantly compared with NC group,the differences were statistically significant (P＜0.05).Conclusion:miR-346 is overexpression in nasopharyngeal carcinoma,down-regu-lation of miR-346 inhibits the proliferation,migration,invasion,promotes the apoptosis,miR-346 may act as a oncogene and play an important role in the pathogenesis of nasopharyngeal carcinoma.
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Objective:To investigate the expression and biological significance of MicroRNA-204 in nasopharyngeal carcinoma (NPC). Methods: qRT-PCR was applied to detect the relative expression of miR-204 in 43 paired nasopharyngeal carcinoma in comparison to the normal nasal mucosa tissues. Pearson chi-square test was used to analyze the relationship between the miR-204 expression and clinical features. The expressions of Bcl-2 and SIRT1 were measured by immunohistochemistry ( IHC ) , Spearman correlation analysis was used to analyze the relationship between miR-204 and Bcl-2,as well as SIRT1. We then transfected the miR-204 mimics into CNE-2 cells,then the Western blot was used to detect the expression of Bcl-2 and SIRT1,which were considered as the potential targets of miR-204. Results:The relative expressions of miR-204 was significantly downregulated in NPC tissues compared to those of the matched normal tumor-adjacent tissues(P<0. 05). Low expression of miR-204 was significantly associated with lymphatic metastasis(P<0. 05) and advanced TNM stage(Ⅲ+Ⅳ,P<0. 05). The expressions of Bcl-2 and Sirt1 in lower miR-204 level group were both higher than in higher miR-204 level group ( P<0. 05 ) . Both the mRNA and protin expression in CNE-2 cells were down-regulated after transfection. Conclusion: Low expression of miR-204 is related to the malignant clinicopathological features in NPC tissues,and miR-204 may through down-regulate Bcl-2 and SIRT1 to suppress NPC genesis and development.
ABSTRACT
Objective To investigate the effect of anti-bleeding gauze (TISTAT) in nasal packing after endoscopic sinus surgery in elderly patients.Methods 72 elderly patients with chronic sinusitis and nasal polyps for endoscopic sinus surgery were divided into two groups:TISTAT group (use TISTAT,n=38) and calcium alginate group(use calcium alginate,n=34).TISTAT and calcium alginate were respectively used as packing materials in TISTAT and calcium alginate group.Hemorrhage in the packing process and after the packing materials removed were observed and compared.The symptoms such as headache、nasal blockage and nose pain during the packing process and 24 hours after the packing materials removed were compared.The recovery of surgery cavity in 4 weeks after surgery which reviewed once a week were checked and compared.Results The tamponade during hemorrhage rates of TISTAT group and calcium alginate group were 31.6％ (12 cases)、58.5％ (20 cases),after removing the tamponade,the total bleeding rates were 15.8％,41.2 ％,two groups had significant difference (P＜0.01) ;TISTAT group nasal pain symptoms in the packing process,packing materials were 12 h,stuffing out after 24 h were (2.8±0.6)、(1.3±0.5),calcium alginate group nasal pain symptoms in the packing process,packing materials were 12 h.stuffing were 24 h were (5.2±0.4)、(4.3±0.6),two groups of nasal pain symptom difference was statistically significant (P＜0.05),but the process of tailing and calcium alginate group blocked nose were (5.4±0.9) 、(8.3±0.6),the two groups had significant difference (P＜0.05).Conclusions The anti-bleeding gauze(TISTAT) has safety and active role in nasal packing after endoscopic sinus surgery in elderly patients with less symptoms and a better surgical cavity recovery.It is an ideal packing material.